Hotspot

tRNA-Gly-tRNA-Ser

Genome · 78959-79654 · 696 bp

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Pi 0.309
MarkerSeek score 80.60
Primer available yes
Label tRNA-Gly-tRNA-Ser

Feature Metadata

label_name
tRNA-Gly-tRNA-Ser
feature_type
igs
region
Genome
length_bp
696
variable_sites
281
pi
0.309
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.87
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Gly-tRNA-Ser_p1 CATCGTTAGCTTGGAAGGCT CAATCCGACGCTTTAGTCCA 50.0 50.0 58.0 58.0 1462 bp 0.333333 53.1
tRNA-Gly-tRNA-Ser_p2 CATCGTTAGCTTGGAAGGCT AATCCGACGCTTTAGTCCAC 50.0 50.0 58.0 58.0 1461 bp 0.333333 53.0
tRNA-Gly-tRNA-Ser_p3 TAGCTTGGAAGGCTAAGGGT CAATCCGACGCTTTAGTCCA 50.0 50.0 58.0 58.0 1456 bp 0.333333 53.0
tRNA-Gly-tRNA-Ser_p4 TAGCTTGGAAGGCTAAGGGT AATCCGACGCTTTAGTCCAC 50.0 50.0 58.0 58.0 1455 bp 0.333333 53.0
tRNA-Gly-tRNA-Ser_p5 CATCGTTAGCTTGGAAGGCT TCTAACGACCCAGGACGTAA 50.0 50.0 58.0 57.8 1697 bp 0.333333 51.5