Hotspot

tRNA-Cys-tRNA-Arg

Genome · 59416-77879 · 18464 bp

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Pi 0.33

MarkerSeek score 72.50

Primer available yes

Label tRNA-Cys-tRNA-Arg

Feature Metadata

label_name: tRNA-Cys-tRNA-Arg
feature_type: igs
region: Genome
length_bp: 18464
variable_sites: 7317
pi: 0.33
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Cys-tRNA-Arg_p1	CGAAGCTTATCCCGGAGATG	GCTTCTGCTTCGCTAGTGAA	55.0	50.0	57.9	58.3	612 bp	0.333333	50.5
tRNA-Cys-tRNA-Arg_p2	CATTACAATACCTCGCGCCT	GCTTCTGCTTCGCTAGTGAA	50.0	50.0	58.1	58.3	763 bp	0.333333	50.0
tRNA-Cys-tRNA-Arg_p3	CAGCAGTAGCCACAGATACG	GCTTCTGCTTCGCTAGTGAA	55.0	50.0	58.2	58.3	934 bp	0.333333	49.8
tRNA-Cys-tRNA-Arg_p4	CGAAGCTTATCCCGGAGATG	CCTTCAAAAGGGCTTCTGCT	55.0	50.0	57.9	58.4	623 bp	0.333333	49.8
tRNA-Cys-tRNA-Arg_p5	TTGGATCAGCGGCTCAAATT	GCTTCTGCTTCGCTAGTGAA	45.0	50.0	58.2	58.3	364 bp	0.333333	49.8