Hotspot

tRNA-Asp-tRNA-Cys

Genome · 57184-59344 · 2161 bp

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Pi 0.345

MarkerSeek score 79.00

Primer available yes

Label tRNA-Asp-tRNA-Cys

Feature Metadata

label_name: tRNA-Asp-tRNA-Cys
feature_type: igs
region: Genome
length_bp: 2161
variable_sites: 962
pi: 0.345
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asp-tRNA-Cys_p1	GAAGTCCGCCTCTTTCAGTT	CTTTTTGTTCGGGACAGCAC	50.0	50.0	57.8	57.9	2968 bp	0.333333	51.0
tRNA-Asp-tRNA-Cys_p2	CCAATGGGCTCGGATACAAA	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.9	58.4	2653 bp	0.333333	50.1
tRNA-Asp-tRNA-Cys_p3	TGCAAAATTCCGATCGGGAT	GGGGGACTGCAAATCCTTTT	45.0	50.0	57.9	58.4	2575 bp	0.333333	50.1
tRNA-Asp-tRNA-Cys_p4	CATAGTAGAATGGCGGTCGG	GGGGGACTGCAAATCCTTTT	55.0	50.0	57.9	58.4	2949 bp	0.333333	50.0
tRNA-Asp-tRNA-Cys_p5	AAGTATGCCCCCATCGTCTA	GGGGGACTGCAAATCCTTTT	50.0	50.0	57.9	58.4	2927 bp	0.333333	49.7