Hotspot

tRNA-Thr-tRNA-Ser

Genome · 44840-45992 · 1153 bp

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Pi 0.329

MarkerSeek score 82.90

Primer available yes

Label tRNA-Thr-tRNA-Ser

Feature Metadata

label_name: tRNA-Thr-tRNA-Ser
feature_type: igs
region: Genome
length_bp: 1153
variable_sites: 526
pi: 0.329
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr-tRNA-Ser_p1	TGTGTTATGAAAGCCCGCTT	GAGGCTGCATTAATCGGGAA	45.0	50.0	58.1	58.0	1885 bp	0.333333	52.3
tRNA-Thr-tRNA-Ser_p2	TTGTGTTATGAAAGCCCGCT	GAGGCTGCATTAATCGGGAA	45.0	50.0	58.1	58.0	1886 bp	0.333333	52.3
tRNA-Thr-tRNA-Ser_p3	CTCAGAGGTTAGAGCATCGC	GAGGCTGCATTAATCGGGAA	55.0	50.0	57.9	58.0	1863 bp	0.333333	52.0
tRNA-Thr-tRNA-Ser_p4	TGTGTTATGAAAGCCCGCTT	CGGGAATACACGACAGAAGG	45.0	55.0	58.1	58.1	1871 bp	0.333333	51.9
tRNA-Thr-tRNA-Ser_p5	TTGTGTTATGAAAGCCCGCT	CGGGAATACACGACAGAAGG	45.0	55.0	58.1	58.1	1872 bp	0.333333	51.9