Hotspot

tRNA-Leu-tRNA-Leu

Genome · 42925-43466 · 542 bp

Back to Trifolium

Pi 0.313

MarkerSeek score 79.80

Primer available yes

Label tRNA-Leu-tRNA-Leu

Feature Metadata

label_name: tRNA-Leu-tRNA-Leu
feature_type: igs
region: Genome
length_bp: 542
variable_sites: 221
pi: 0.313
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu-tRNA-Leu_p1	TTTGAACTGGTGACACGAGG	GGGGATATGGCGAAATTGGT	50.0	50.0	58.1	58.0	816-833 bp	1.0	91.5
tRNA-Leu-tRNA-Leu_p2	TGAGCTATCCCGACCATTCT	GGGGATATGGCGAAATTGGT	50.0	50.0	57.9	58.0	771-788 bp	1.0	91.3
tRNA-Leu-tRNA-Leu_p3	TACAAACGAATTGCCGGGAA	GGGGATATGGCGAAATTGGT	45.0	50.0	58.1	58.0	841-858 bp	1.0	91.1
tRNA-Leu-tRNA-Leu_p4	TTTGAACTGGTGACACGAGG	GGCGAAATTGGTAGACGCTA	50.0	50.0	58.1	58.1	808-825 bp	1.0	91.0
tRNA-Leu-tRNA-Leu_p5	ATACAAACGAATTGCCGGGA	GGGGATATGGCGAAATTGGT	45.0	50.0	57.9	58.0	842-859 bp	1.0	91.0