Hotspot

tRNA-Lys-tRNA-His

Genome · 8528-10339 · 1812 bp

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Pi 0.337
MarkerSeek score 77.70
Primer available yes
Label tRNA-Lys-tRNA-His

Feature Metadata

label_name
tRNA-Lys-tRNA-His
feature_type
igs
region
Genome
length_bp
1812
variable_sites
732
pi
0.337
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.80
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys-tRNA-His_p1 GGGCATCCCATTAGTAAGCC CAATCCACTGCCTTGATCCA 55.0 50.0 58.1 57.9 2451 bp 0.333333 51.6
tRNA-Lys-tRNA-His_p2 GGGCATCCCATTAGTAAGCC AATCCACTGCCTTGATCCAC 55.0 50.0 58.1 57.9 2450 bp 0.333333 51.5
tRNA-Lys-tRNA-His_p3 GGGCATCCCATTAGTAAGCC ACAATCCACTGCCTTGATCC 55.0 50.0 58.1 57.9 2452 bp 0.333333 51.5
tRNA-Lys-tRNA-His_p4 TGGTACGGAGTCAAATGCTG CAATCCACTGCCTTGATCCA 50.0 50.0 57.9 57.9 2576 bp 0.333333 51.5
tRNA-Lys-tRNA-His_p5 GTGGTACGGAGTCAAATGCT CAATCCACTGCCTTGATCCA 50.0 50.0 57.9 57.9 2577 bp 0.333333 51.5