Hotspot

tRNA-Val-tRNA-Met

Genome · 958-1133 · 176 bp

Back to Trifolium

Pi 0.336

MarkerSeek score 77.60

Primer available yes

Label tRNA-Val-tRNA-Met

Feature Metadata

label_name: tRNA-Val-tRNA-Met
feature_type: igs
region: Genome
length_bp: 176
variable_sites: 77
pi: 0.336
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Val-tRNA-Met_p1	GTGTGTAAACGAGGTGCTCT	TCCTGCCGTATGAAAGCAAT	50.0	45.0	57.8	57.6	262 bp	0.333333	48.8
tRNA-Val-tRNA-Met_p2	CGTGTGTAAACGAGGTGCT	TCCTGCCGTATGAAAGCAAT	52.6	45.0	58.1	57.6	263 bp	0.333333	41.1
tRNA-Val-tRNA-Met_p3	CGTGTGTAAACGAGGTGCTC	TCCTGCCGTATGAAAGCAAT	55.0	45.0	59.2	57.6	263 bp	0.333333	40.2
tRNA-Val-tRNA-Met_p4	GTGTGTAAACGAGGTGCTCT	TCCTGCCGTATGAAAGCAATA	50.0	42.9	57.8	57.4	262 bp	0.333333	39.7
tRNA-Val-tRNA-Met_p5	TGTGTAAACGAGGTGCTCTAC	TCCTGCCGTATGAAAGCAAT	47.6	45.0	57.7	57.6	261 bp	0.333333	39.6