Hotspot

23S ribosomal RNA

Genome · 106084-108882 · 2799 bp

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Pi 0.349
MarkerSeek score 77.00
Primer available yes
Label 23S ribosomal RNA

Feature Metadata

label_name
23S ribosomal RNA
feature_type
rRNA
region
Genome
length_bp
2799
variable_sites
1223
pi
0.349
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.84
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
23S ribosomal RNA_p1 CCGTGACCTTCTCTTGCATT CCATCTAGCAGCACCTTAGG 50.0 55.0 58.2 57.5 2957 bp 0.333333 47.5
23S ribosomal RNA_p2 CCGTGACCTTCTCTTGCATT CTAGCAGCACCTTAGGATGG 50.0 55.0 58.2 57.5 2953 bp 0.333333 47.5
23S ribosomal RNA_p3 AACTTCTTTTGCTCCACCCC CCATCTAGCAGCACCTTAGG 50.0 55.0 58.3 57.5 2932 bp 0.333333 46.6
23S ribosomal RNA_p4 AACTTCTTTTGCTCCACCCC CTAGCAGCACCTTAGGATGG 50.0 55.0 58.3 57.5 2928 bp 0.333333 46.6
23S ribosomal RNA_p5 CCGTGACCTTCTCTTGCATT CTTCCATCTAGCAGCACCTT 50.0 50.0 58.2 57.3 2960 bp 0.333333 46.2