Hotspot

16S ribosomal RNA

Genome · 111071-112563 · 1493 bp

Back to Trifolium

Pi 0.342

MarkerSeek score 82.20

Primer available yes

Label 16S ribosomal RNA

Feature Metadata

label_name: 16S ribosomal RNA
feature_type: rRNA
region: Genome
length_bp: 1493
variable_sites: 700
pi: 0.342
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
16S ribosomal RNA_p1	TCTCTTTCCCCCTTTTTGCC	TGGATAAGAGGCTTGTGGGA	50.0	50.0	58.0	58.0	1921 bp	0.333333	53.0
16S ribosomal RNA_p2	TCTTTCCCCCTTTTTGCCTC	TGGATAAGAGGCTTGTGGGA	50.0	50.0	58.0	58.0	1919 bp	0.333333	53.0
16S ribosomal RNA_p3	GAGAGGAGAGGAGCCATGAT	TGGATAAGAGGCTTGTGGGA	55.0	50.0	58.0	58.0	2277 bp	0.333333	52.9
16S ribosomal RNA_p4	CTCTTTCCCCCTTTTTGCCT	TGGATAAGAGGCTTGTGGGA	50.0	50.0	58.0	58.0	1920 bp	0.333333	52.9
16S ribosomal RNA_p5	AGCTAATAGCCCGTTGTGTG	TGGATAAGAGGCTTGTGGGA	50.0	50.0	58.0	58.0	1990 bp	0.333333	52.9