Hotspot

atpF

LSC · 12340-13664 · 1325 bp

Back to Tricyrtis

Pi 0.0143

MarkerSeek score 57.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1325
variable_sites: 19
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CTCGAAGGGTTGCCATTAGT	GGCGAGTGAGGTGATACAAA	50.0	50.0	57.6	57.6	1487 bp	1.0	74.8
atpF_p2	ACTAATTTCGTCGGCTCGAA	GGCGAGTGAGGTGATACAAA	45.0	50.0	57.4	57.6	1501 bp	1.0	72.8
atpF_p3	GCTCGAAGGGTTGCCATTAG	GGCGAGTGAGGTGATACAAA	55.0	50.0	59.0	57.6	1488 bp	1.0	70.1
atpF_p4	GCTCGAAGGGTTGCCATTA	GGCGAGTGAGGTGATACAAA	52.6	50.0	57.8	57.6	1488 bp	1.0	68.6
atpF_p5	CTCGAAGGGTTGCCATTAGT	GGCGAGTGAGGTGATACAAAA	50.0	47.6	57.6	58.3	1487 bp	1.0	67.4