Hotspot

psbT-psbN

LSC · 75052-75120 · 69 bp

Back to Tricyrtis

Pi 0.0725

MarkerSeek score 81.40

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 5
pi: 0.0725
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACCACCTAAGGTTCCGACTA	ATCCATTCGAAGAACACGGG	50.0	50.0	57.4	58.0	123 bp	1.0	78.2
psbT-psbN_p2	ACCACCTAAGGTTCCGACTA	CGCCATCTTCATATCCGGTT	50.0	50.0	57.4	57.8	209 bp	1.0	75.7
psbT-psbN_p3	ACCACCTAAGGTTCCGACTA	TTCGAAGAACACGGGGACTA	50.0	50.0	57.4	58.4	118 bp	1.0	75.5
psbT-psbN_p4	ACCACCTAAGGTTCCGACTA	GGGTACGCCTTATATACCGC	50.0	55.0	57.4	57.7	172 bp	1.0	75.2
psbT-psbN_p5	ACCACCTAAGGTTCCGACTA	GTCGCCATCTTCATATCCGG	50.0	55.0	57.4	58.3	211 bp	1.0	74.7