Hotspot

psaJ-rpl33

LSC · 67717-68251 · 535 bp

Back to Tricyrtis

Pi 0.015

MarkerSeek score 66.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 535
variable_sites: 8
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACGCCTTGTCATTTCCCTTT	CCATTTCGGGCACAACAATT	45.0	45.0	58.0	57.5	635-637 bp	1.0	77.3
psaJ-rpl33_p2	ACGCCTTGTCATTTCCCTTT	TATTGACACCATTTCGGGCA	45.0	45.0	58.0	57.5	643-645 bp	1.0	77.0
psaJ-rpl33_p3	ACGCCTTGTCATTTCCCTTT	GACATCTTTACCCTTGGCCA	45.0	50.0	58.0	57.5	588-590 bp	1.0	77.0
psaJ-rpl33_p4	ACATATCTTTCCACGGCACC	CCATTTCGGGCACAACAATT	50.0	45.0	58.0	57.5	723-725 bp	1.0	76.9
psaJ-rpl33_p5	ATCGTTTATTCCCAGACGCC	CCATTTCGGGCACAACAATT	50.0	45.0	58.1	57.5	650-652 bp	1.0	76.9