Hotspot

psaI-ycf4

LSC · 59938-60302 · 365 bp

Back to Tricyrtis

Pi 0.0192

MarkerSeek score 50.60

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 365
variable_sites: 7
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	CCCGCGATCCCATAAAAAGA	45.0	50.0	58.0	58.0	644-658 bp	1.0	80.8
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	CATTACGATCCCTTGCGGAA	45.0	50.0	58.0	58.1	624-638 bp	1.0	80.8
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	ACGCGCAAGAAATAGGCTAA	45.0	45.0	58.0	58.0	962-976 bp	1.0	80.5
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CACTACCTACATTCCACGCC	45.0	55.0	58.0	58.1	697-711 bp	1.0	80.5
psaI-ycf4_p5	TGGATGGGATTAGTGCAAGC	ACGCGCAAGAAATAGGCTAA	50.0	45.0	57.9	58.0	1298 bp	0.5	59.4