Hotspot

rbcL-accD

LSC · 56648-57468 · 821 bp

Back to Tricyrtis

Pi 0.00993

MarkerSeek score 65.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 8
pi: 0.00993
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGCAAGTGGAGTCCTGAAC	GGTTCTTCACTTCCACTGCT	50.0	50.0	58.0	57.7	1026-1040 bp	1.0	79.4
rbcL-accD_p2	GTTCGAGCCGGTAGATAAGC	GGTTCTTCACTTCCACTGCT	55.0	50.0	58.2	57.7	966-980 bp	1.0	77.9
rbcL-accD_p3	CTTGCAAGTGGAGTCCTGAA	GGTTCTTCACTTCCACTGCT	50.0	50.0	57.7	57.7	1027-1041 bp	1.0	77.6
rbcL-accD_p4	TTCGAGCCGGTAGATAAGCT	GGTTCTTCACTTCCACTGCT	50.0	50.0	58.3	57.7	965-979 bp	1.0	77.3
rbcL-accD_p5	TGAAGCTTGCAAGTGGAGTC	GGTTCTTCACTTCCACTGCT	50.0	50.0	58.4	57.7	1032-1046 bp	1.0	76.5