Hotspot

atpB-rbcL

LSC · 54444-55183 · 740 bp

Back to Tricyrtis

Pi 0.00952

MarkerSeek score 64.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 7
pi: 0.00952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AATGACATCCAATACCGGGC	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.0	58.1	871-879 bp	1.0	80.0
atpB-rbcL_p2	CGGGGGAAAAATGACATCCA	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.2	58.1	880-888 bp	1.0	78.9
atpB-rbcL_p3	AATGACATCCAATACCGGGC	GCTTTGAATCCAGCACTTGC	50.0	50.0	58.0	58.3	907-915 bp	1.0	78.6
atpB-rbcL_p4	AATGACATCCAATACCGGGC	TGGTGACATAAGTCCCTCCC	50.0	55.0	58.0	58.4	872-880 bp	1.0	77.6
atpB-rbcL_p5	CGGGGGAAAAATGACATCCA	GCTTTGAATCCAGCACTTGC	50.0	50.0	58.2	58.3	916-924 bp	1.0	77.5