Hotspot

ndhC-trnV-UAC

LSC · 50875-51418 · 544 bp

Back to Tricyrtis

Pi 0.0292

MarkerSeek score 78.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 544
variable_sites: 15
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTCTGGCCCTTCACTAATCG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	57.8	58.1	697-705 bp	1.0	82.5
ndhC-trnV-UAC_p2	AATCGGTGCTAAAACTCCGG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.3	58.1	682-690 bp	1.0	82.4
ndhC-trnV-UAC_p3	CTCTGGCCCTTCACTAATCG	AGCCCAATGCAAGAAAATGC	55.0	45.0	57.8	57.9	776-784 bp	1.0	82.0
ndhC-trnV-UAC_p4	AATCGGTGCTAAAACTCCGG	AGCCCAATGCAAGAAAATGC	50.0	45.0	58.3	57.9	761-769 bp	1.0	81.9
ndhC-trnV-UAC_p5	CGGTGCTAAAACTCCGGAAA	AGCCCAATGCAAGAAAATGC	50.0	45.0	58.5	57.9	758-766 bp	1.0	80.1