Hotspot

trnT-UGU-trnL-UAA

LSC · 46802-47765 · 964 bp

Back to Tricyrtis

Pi 0.0126

MarkerSeek score 64.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 964
variable_sites: 12
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	1025-1071 bp	1.0	78.9
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	1057-1103 bp	1.0	78.0
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	1019-1065 bp	1.0	77.6
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	1051-1097 bp	1.0	76.7
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GCCCATTTTTAATTCCGGGG	55.0	50.0	57.9	57.4	1108-1154 bp	1.0	75.9