Hotspot

ycf1

SSC · 126149-131686 · 5538 bp

Back to Trichosanthes

Pi 0.012

MarkerSeek score 49.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5538
variable_sites: 242
pi: 0.012
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGATTGTTGGGAATACCGT	CTAGCTCGGGATGTACTGGA	50.0	55.0	58.0	58.0	577 bp	1.0	85.1
ycf1_p2	CCGATTGTTGGGAATACCGT	TCTAGCTCGGGATGTACTGG	50.0	55.0	58.0	58.0	578 bp	1.0	85.1
ycf1_p3	CCGATTGTTGGGAATACCGT	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.0	58.0	1192-1198 bp	1.0	83.9
ycf1_p4	CCGATTGTTGGGAATACCGT	ACAGGAACAAGAGGGATCCA	50.0	50.0	58.0	58.0	1178-1184 bp	1.0	83.7
ycf1_p5	CCGATTGTTGGGAATACCGT	AAGAGGGATCCACCGAAGAA	50.0	50.0	58.0	58.0	1170-1176 bp	1.0	83.7