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psbE-petL

LSC · 66513-67742 · 1230 bp

Back to Trichosanthes

Pi 0.0154

MarkerSeek score 63.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 67
pi: 0.0154
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AACTGCTAGTAGAAAACCGA	50.0	40.0	57.8	54.0	907-1368 bp	1.0	55.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	ACTGCTAGTAGAAAACCGAA	50.0	40.0	57.8	54.0	906-1367 bp	1.0	55.3
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AACTGCTAGTAGAAAACCGA	55.0	40.0	58.2	54.0	902-1363 bp	1.0	55.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	ACTGCTAGTAGAAAACCGAA	55.0	40.0	58.2	54.0	901-1362 bp	1.0	55.0
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	ACTGCTAGTAGAAAACCGAAA	50.0	38.1	57.8	54.9	906-1367 bp	1.0	54.2