Hotspot

trnF-ndhJ

LSC · 50683-51378 · 696 bp

Back to Trichosanthes

Pi 0.019

MarkerSeek score 70.50

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 45
pi: 0.019
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGGTAGGATGGCC	50.0	55.0	58.1	58.2	803-860 bp	1.0	86.0
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGTAGGATGGCCCTTACGTA	50.0	55.0	58.1	58.3	795-852 bp	1.0	85.6
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGGTAGGATGGC	50.0	50.0	58.1	57.5	804-861 bp	1.0	83.8
trnF-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCTGAAAGTTGGGTAGG	50.0	50.0	58.1	57.5	809-866 bp	1.0	83.8
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGCCTGAAAGTTGGGTAGGA	50.0	50.0	58.1	58.6	808-865 bp	1.0	83.4