Hotspot

psbZ-trnG

LSC · 37965-38502 · 538 bp

Back to Trichosanthes

Pi 0.0256

MarkerSeek score 71.50

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 538
variable_sites: 40
pi: 0.0256
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	CTTCTCCTGATGGTTGGTCG	AATCGAACCCGCATCTTCTC	55.0	50.0	58.0	58.1	615-714 bp	1.0	88.2
psbZ-trnG_p2	CTTCTCCTGATGGTTGGTCG	GAATCGAACCCGCATCTTCT	55.0	50.0	58.0	58.1	616-715 bp	1.0	88.2
psbZ-trnG_p3	GATGGTTGGTCGGGTAACAA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	607-706 bp	1.0	86.9
psbZ-trnG_p4	GATGGTTGGTCGGGTAACAA	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	608-707 bp	1.0	86.9
psbZ-trnG_p5	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	618-717 bp	1.0	86.6