Hotspot

trnC-petN

LSC · 29191-30091 · 901 bp

Back to Trichosanthes

Pi 0.0203

MarkerSeek score 74.90

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 60
pi: 0.0203
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1007-1039 bp	1.0	84.1
trnC-petN_p2	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1064-1096 bp	1.0	83.9
trnC-petN_p3	TGCAAATCCCTTTTTCCCCA	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1019-1051 bp	1.0	83.8
trnC-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	954-986 bp	1.0	80.1
trnC-petN_p5	TTTTGTATCGTTTTGGCGGC	ATTAAAGCAGCCCAAGCGAG	45.0	50.0	57.9	58.9	1011-1043 bp	1.0	79.8