Hotspot

rps16-trnQ

LSC · 6250-7572 · 1323 bp

Back to Trichosanthes

Pi 0.0202

MarkerSeek score 76.00

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1323
variable_sites: 111
pi: 0.0202
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1332-1430 bp	1.0	88.7
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1343-1441 bp	1.0	88.1
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	GACGACCAACCACTACCAAT	45.0	50.0	58.1	57.5	1428-1526 bp	1.0	85.2
rps16-trnQ_p4	TCATGTCCTTCAAGTCGCAC	GACGACCAACCACTACCAAT	50.0	50.0	58.2	57.5	1439-1537 bp	1.0	84.7
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1336-1434 bp	1.0	83.9