Hotspot

ndhD

SSC · 106514-108016 · 1503 bp

Back to Trichoneura

Pi 0.00235

MarkerSeek score 58.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 10
pi: 0.00235
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAGACGTTCAAAGGGGTTCC	GGGCTTTTCTGGTCAAAACG	50.0	50.0	58.0	57.9	1662-1663 bp	1.0	79.2
ndhD_p2	AAGACGTTCAAAGGGGTTCC	TTTACCGAAGAAGCCTGTGC	50.0	50.0	58.0	58.5	1699-1700 bp	1.0	76.4
ndhD_p3	AAGACGTTCAAAGGGGTTCC	GGCTTTTCTGGTCAAAACGT	50.0	45.0	58.0	57.1	1661-1662 bp	1.0	73.4
ndhD_p4	AAGACGTTCAAAGGGGTTCC	GCATGGGCTTTTCTGGTCAA	50.0	50.0	58.0	59.0	1666-1667 bp	1.0	72.0
ndhD_p5	AAGACGTTCAAAGGGGTTCC	TCTTTACCGAAGAAGCCTGTG	50.0	47.6	58.0	58.0	1701-1702 bp	1.0	72.0