Hotspot

ndhF-rpl32

SSC · 103734-104624 · 891 bp

Back to Trichoneura

Pi 0.00667
MarkerSeek score 53.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
891
variable_sites
17
pi
0.00667
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 973-975 bp 1.0 42.5
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 974-976 bp 1.0 42.5
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 975-977 bp 1.0 42.5
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 976-978 bp 1.0 42.5
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATCGAAGTACGT 55.0 35.0 58.1 53.2 977-979 bp 1.0 42.5