Hotspot

atpB-rbcL

LSC · 54237-55039 · 803 bp

Back to Trichoneura

Pi 0.00407

MarkerSeek score 56.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 9
pi: 0.00407
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTCAATTGTGGAAACCCCGG	GCTTGAAATCCAACACCTGC	50.0	50.0	58.7	57.9	881-895 bp	1.0	77.1
atpB-rbcL_p2	CTTCAATTGTGGAAACCCCG	GCTTGAAATCCAACACCTGC	50.0	50.0	57.3	57.9	882-896 bp	1.0	76.6
atpB-rbcL_p3	TCAATTGTGGAAACCCCGG	GCTTGAAATCCAACACCTGC	52.6	50.0	58.0	57.9	880-894 bp	1.0	74.1
atpB-rbcL_p4	AGCACGGGTCCAATAATTTGA	GCTTGAAATCCAACACCTGC	42.9	50.0	57.9	57.9	925-939 bp	1.0	73.6
atpB-rbcL_p5	GGGACGCGAAGTAGTAGAAT	GCTTGAAATCCAACACCTGC	50.0	50.0	56.8	57.9	863-877 bp	1.0	73.2