Hotspot

trnT-UGU-trnL-UAA

LSC · 46167-46993 · 827 bp

Back to Trichoneura

Pi 0.00738

MarkerSeek score 60.50

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 17
pi: 0.00738
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	903-928 bp	1.0	83.6
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	895-920 bp	1.0	83.3
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	873-898 bp	1.0	82.9
trnT-UGU-trnL-UAA_p4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	893-918 bp	1.0	81.7
trnT-UGU-trnL-UAA_p5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.0	57.7	902-927 bp	1.0	81.4