Hotspot

ycf3-trnS-GGA

LSC · 44257-44835 · 579 bp

Back to Trichoneura

Pi 0.00484

MarkerSeek score 58.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 7
pi: 0.00484
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	726-780 bp	1.0	83.3
ycf3-trnS-GGA_p2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	676-730 bp	1.0	83.1
ycf3-trnS-GGA_p3	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.0	58.5	678-732 bp	1.0	80.2
ycf3-trnS-GGA_p4	TTTTTCCCCTGAGGTTGTCG	CAGTTCCAATGCTACGCCTT	50.0	50.0	58.0	58.5	679-733 bp	1.0	80.2
ycf3-trnS-GGA_p5	TTTTTCCCCTGAGGTTGTCG	TCCAATGCTACGCCTTGAAC	50.0	50.0	58.0	58.6	675-729 bp	1.0	80.2