Hotspot

psaA-ycf3

LSC · 41611-42286 · 676 bp

Back to Trichoneura

Pi 0.00444

MarkerSeek score 63.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 9
pi: 0.00444
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	864-872 bp	1.0	81.3
psaA-ycf3_p2	GGGGCCCTTAGCTATTGTTC	GCGCTTACTCCGGGAAATTA	55.0	50.0	58.0	58.1	860-868 bp	1.0	81.0
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	829-837 bp	1.0	80.9
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	825-833 bp	1.0	80.6
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	827-835 bp	1.0	79.8