Hotspot

trnC-GCA-rpoB

LSC · 18502-19500 · 999 bp

Back to Trichoneura

Pi 0.00337

MarkerSeek score 61.90

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 10
pi: 0.00337
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	GTGGACATTCCCTCATTCCC	50.0	55.0	58.1	57.9	1057-1074 bp	1.0	81.1
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGGACATTCCCTCATTCCCA	50.0	50.0	58.1	58.3	1056-1073 bp	1.0	79.0
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1066-1083 bp	1.0	78.9
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1065-1082 bp	1.0	78.9
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1130-1147 bp	1.0	78.4