Hotspot

trnY-GUA-trnD-GUC

LSC · 15707-16057 · 351 bp

Back to Trichoneura

Pi 0.0114
MarkerSeek score 49.90
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
351
variable_sites
12
pi
0.0114
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC GCGGTGCTCTAACCAATTGA 50.0 50.0 58.0 58.6 476 bp 1.0 77.3
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG GCGGTGCTCTAACCAATTGA 55.0 50.0 58.4 58.6 443 bp 1.0 74.9
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT GCGGTGCTCTAACCAATTGA 50.0 50.0 57.6 58.6 442 bp 1.0 74.8
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGCGGTGCTCTAACCAATT 50.0 52.6 58.0 58.1 477 bp 1.0 72.6
trnY-GUA-trnD-GUC_p5 TGGGGACGGACTGTAAATTC GCGGTGCTCTAACCAATTGA 50.0 50.0 57.2 58.6 444 bp 1.0 71.6