Hotspot

trnG-UCC-trnE-UUC

LSC · 13883-15488 · 1606 bp

Back to Trichoneura

Pi 0.0057

MarkerSeek score 58.00

Primer available yes

Label trnG-UCC-trnE-UUC

Feature Metadata

label_name: trnG-UCC-trnE-UUC
feature_type: igs
region: LSC
length_bp: 1606
variable_sites: 25
pi: 0.0057
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnE-UUC_p1	ACGAATCACACTTTTACCACT	GCTGCCTCCTTGAAAGAGAG	38.1	55.0	55.5	58.3	1661-1688 bp	1.0	52.3
trnG-UCC-trnE-UUC_p2	ACGAATCACACTTTTACCACT	TGTCCTGAACCACTAGACGA	38.1	50.0	55.5	57.7	1640-1667 bp	1.0	52.2
trnG-UCC-trnE-UUC_p3	AGAACGAATCACACTTTTACCAC	GCTGCCTCCTTGAAAGAGAG	39.1	55.0	57.3	58.3	1664-1691 bp	1.0	51.3
trnG-UCC-trnE-UUC_p4	GAACGAATCACACTTTTACCACT	GCTGCCTCCTTGAAAGAGAG	39.1	55.0	57.3	58.3	1663-1690 bp	1.0	51.3
trnG-UCC-trnE-UUC_p5	GAACGAATCACACTTTTACCACT	TGTCCTGAACCACTAGACGA	39.1	50.0	57.3	57.7	1642-1669 bp	1.0	51.2