Hotspot

trnS-GCU-psbD

LSC · 8220-9181 · 962 bp

Back to Trichoneura

Pi 0.00568

MarkerSeek score 58.20

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 15
pi: 0.00568
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1106-1113 bp	1.0	79.6
trnS-GCU-psbD_p2	GAAAGTCCCAAACGATCCGA	GCCGGACCATCCTACAAAAA	50.0	50.0	57.9	58.2	1179-1186 bp	1.0	79.1
trnS-GCU-psbD_p3	TCGAAAGTCCCAAACGATCC	GCCGGACCATCCTACAAAAA	50.0	50.0	57.9	58.2	1181-1188 bp	1.0	79.1
trnS-GCU-psbD_p4	AAAGTCCCAAACGATCCGAG	GCCGGACCATCCTACAAAAA	50.0	50.0	57.9	58.2	1178-1185 bp	1.0	79.0
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1093-1100 bp	1.0	79.0