Hotspot

rps16-trnQ-UUG

LSC · 5590-6853 · 1264 bp

Back to Trichoneura

Pi 0.00486
MarkerSeek score 60.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1264
variable_sites
17
pi
0.00486
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1336-1353 bp 1.0 79.9
rps16-trnQ-UUG_p2 TCATCTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1347-1364 bp 1.0 78.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1340-1357 bp 1.0 75.1
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1328-1345 bp 1.0 74.7
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1335-1352 bp 1.0 74.1