Hotspot

ycf2

Genome · 1-5485 · 5485 bp

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Pi 0.023

MarkerSeek score 55.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 5485
variable_sites: 282
pi: 0.023
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GTATGCCTGTCTTCGTCTCG	TCTTCTGGGCCCAATTCAAG	55.0	50.0	58.2	57.8	1369-1378 bp	1.0	82.7
ycf2_p2	GTATGCCTGTCTTCGTCTCG	ATCTATCCGTTAGCTGGGCA	55.0	50.0	58.2	58.3	1207-1216 bp	1.0	82.4
ycf2_p3	GTATGCCTGTCTTCGTCTCG	AGTTCTATCTTCTGGGCCCA	55.0	50.0	58.2	57.7	1376-1385 bp	1.0	82.3
ycf2_p4	CCAGAAGATCCAACGAACCC	TCTTCTGGGCCCAATTCAAG	55.0	50.0	58.3	57.8	1439-1448 bp	1.0	82.0
ycf2_p5	CCAGAAGATCCAACGAACCC	AGTTCTATCTTCTGGGCCCA	55.0	50.0	58.3	57.7	1446-1455 bp	1.0	81.6