Hotspot

infA-rps8

Genome · 84416-84487 · 72 bp

Back to Trichomanes

Pi 0.05

MarkerSeek score 68.10

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: Genome
length_bp: 72
variable_sites: 8
pi: 0.05
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ACCTTCTACATCAATCAGATTCTGT	CAGATCGCGAGGCTAGAAAA	36.0	50.0	57.8	57.8	164-170 bp	1.0	43.9
infA-rps8_p2	ACCTTCTACATCAATCAGATTCTGT	TATGACAGATCGCGAGGCTA	36.0	50.0	57.8	58.1	169-175 bp	1.0	43.8
infA-rps8_p3	ACTGTACCTTCTACATCAATCAGA	CAGATCGCGAGGCTAGAAAA	37.5	50.0	56.9	57.8	169-175 bp	1.0	43.8
infA-rps8_p4	ACTGTACCTTCTACATCAATCAGA	TATGACAGATCGCGAGGCTA	37.5	50.0	56.9	58.1	174-180 bp	1.0	43.7
infA-rps8_p5	ACTGTACCTTCTACATCAATCAGA	AGGTATTAGGCGGTATGGGT	37.5	50.0	56.9	57.6	223-229 bp	1.0	42.9