Hotspot

rps12-clpP

Genome · 74334-74630 · 297 bp

Back to Trichomanes

Pi 0.0392

MarkerSeek score 67.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 297
variable_sites: 24
pi: 0.0392
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTCCATGCGTTGTCTAGCTT	GTACAAAGAACCGGCAAAGC	50.0	50.0	58.0	58.0	486-489 bp	1.0	84.9
rps12-clpP_p2	CTCCATGCGTTGTCTAGCTT	CAGGGGAATGCATCATGGAA	50.0	50.0	58.0	57.9	551-554 bp	1.0	84.3
rps12-clpP_p3	CTCCATGCGTTGTCTAGCTT	AATTGCCTGTACGGTTACCC	50.0	50.0	58.0	57.9	644-647 bp	1.0	83.9
rps12-clpP_p4	CTCCATGCGTTGTCTAGCTT	AAACTATGCCTTCGCCCAAT	50.0	45.0	58.0	57.9	1086-1089 bp	1.0	82.9
rps12-clpP_p5	CTCCATGCGTTGTCTAGCTT	AACTATGCCTTCGCCCAATT	50.0	45.0	58.0	57.9	1085-1088 bp	1.0	82.9