Hotspot

ndhC-trnV-TAC

Genome · 56735-57206 · 472 bp

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Pi 0.0362

MarkerSeek score 62.10

Primer available yes

Label ndhC-trnV-TAC

Feature Metadata

label_name: ndhC-trnV-TAC
feature_type: igs
region: Genome
length_bp: 472
variable_sites: 37
pi: 0.0362
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-TAC_p1	AATTGGACCCAGGTTTCACC	ACGAAAGAAGCATGTTGGGT	50.0	45.0	58.0	58.0	746-751 bp	1.0	85.2
ndhC-trnV-TAC_p2	TCCCTGAAACTCATAGCCCA	ACGAAAGAAGCATGTTGGGT	50.0	45.0	58.0	58.0	836-841 bp	1.0	84.6
ndhC-trnV-TAC_p3	GTCTAGGGCTAGATCGTGGT	ACGAAAGAAGCATGTTGGGT	55.0	45.0	58.0	58.0	1203-1208 bp	1.0	84.2
ndhC-trnV-TAC_p4	TGTCTAGGGCTAGATCGTGG	ACGAAAGAAGCATGTTGGGT	55.0	45.0	58.0	58.0	1204-1209 bp	1.0	84.2
ndhC-trnV-TAC_p5	GACGGAGCCATCTTCATGTT	ACGAAAGAAGCATGTTGGGT	50.0	45.0	58.0	58.0	1255-1260 bp	1.0	83.9