Hotspot

trnS-GGA-rps4

Genome · 52073-52427 · 355 bp

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Pi 0.0293

MarkerSeek score 68.90

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 355
variable_sites: 20
pi: 0.0293
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ACTCCTGTTTACCGAGGGTT	TGCGAATCGAGAATCCATTGA	50.0	42.9	58.3	57.8	402-466 bp	1.0	76.0
trnS-GGA-rps4_p2	CTCCTGTTTACCGAGGGTTC	TGCGAATCGAGAATCCATTGA	55.0	42.9	57.6	57.8	401-465 bp	1.0	75.1
trnS-GGA-rps4_p3	GAAAGGTGACTGAGCGGTTT	TGCGAATCGAGAATCCATTGA	50.0	42.9	58.4	57.8	450-514 bp	1.0	74.6
trnS-GGA-rps4_p4	AGACTCCTGTTTACCGAGGG	TGCGAATCGAGAATCCATTGA	55.0	42.9	58.4	57.8	404-468 bp	1.0	74.6
trnS-GGA-rps4_p5	ACTCCTGTTTACCGAGGGTT	GGAATTGCGAATCGAGAATCC	50.0	47.6	58.3	57.5	407-471 bp	1.0	73.1