Hotspot

trnT-GGT-trnfM-CAT

Genome · 43613-44021 · 409 bp

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Pi 0.0311
MarkerSeek score 66.70
Primer available yes
Label trnT-GGT-trnfM-CAT

Feature Metadata

label_name
trnT-GGT-trnfM-CAT
feature_type
igs
region
Genome
length_bp
409
variable_sites
30
pi
0.0311
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGT-trnfM-CAT_p1 GAACCGATGACTTACGCCTT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.0 58.0 489-509 bp 1.0 88.5
trnT-GGT-trnfM-CAT_p2 GAACCGATGACTTACGCCTT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 483-503 bp 1.0 88.0
trnT-GGT-trnfM-CAT_p3 GAACCGATGACTTACGCCTT TCACGGGTTCAAATCCTGTC 50.0 50.0 58.0 57.8 482-502 bp 1.0 87.4
trnT-GGT-trnfM-CAT_p4 GAACCGATGACTTACGCCTT TGAGGTCACGGGTTCAAATC 50.0 50.0 58.0 57.8 487-507 bp 1.0 87.3
trnT-GGT-trnfM-CAT_p5 CCGATGACTTACGCCTTACC CTTGAGGTCACGGGTTCAAA 55.0 50.0 58.2 58.0 486-506 bp 1.0 87.3