Hotspot

psbD-trnT-GGT

Genome · 42165-43540 · 1376 bp

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Pi 0.0294
MarkerSeek score 71.40
Primer available yes
Label psbD-trnT-GGT

Feature Metadata

label_name
psbD-trnT-GGT
feature_type
igs
region
Genome
length_bp
1376
variable_sites
91
pi
0.0294
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGT_p1 CTCTTCTTAGCCAATCGTCCA GGCGTAAGTCATCGGTTCAA 47.6 50.0 57.5 58.3 1480-1498 bp 1.0 70.8
psbD-trnT-GGT_p2 TCTTCTTAGCCAATCGTCCA GGCGTAAGTCATCGGTTCAA 45.0 50.0 56.3 58.3 1479-1497 bp 1.0 69.1
psbD-trnT-GGT_p3 TCTCTTCTTAGCCAATCGTCC GGCGTAAGTCATCGGTTCAA 47.6 50.0 57.2 58.3 1481-1499 bp 1.0 68.6
psbD-trnT-GGT_p4 TTTTCCAATGGCAATGGTCA GGCGTAAGTCATCGGTTCAA 40.0 50.0 56.1 58.3 1425-1443 bp 1.0 67.9
psbD-trnT-GGT_p5 CTCTTCTTAGCCAATCGTCCA GCCATGGTAAGGCGTAAGTC 47.6 55.0 57.5 58.7 1490-1508 bp 1.0 67.4