Hotspot

trnY-GTA-trnE-TTC

Genome · 36848-37246 · 399 bp

Back to Trichomanes

Pi 0.0498

MarkerSeek score 73.60

Primer available yes

Label trnY-GTA-trnE-TTC

Feature Metadata

label_name: trnY-GTA-trnE-TTC
feature_type: igs
region: Genome
length_bp: 399
variable_sites: 35
pi: 0.0498
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GTA-trnE-TTC_p1	TGGATTTGAACCAGCGTAGG	CTCTCTTTCAAGGAGCCGAC	50.0	55.0	57.9	58.0	381-516 bp	1.0	89.7
trnY-GTA-trnE-TTC_p2	ATTTGAACCAGCGTAGGCAT	CTCTCTTTCAAGGAGCCGAC	45.0	55.0	57.9	58.0	378-513 bp	1.0	89.5
trnY-GTA-trnE-TTC_p3	TGGATTTGAACCAGCGTAGG	TCTCTCTTTCAAGGAGCCGA	50.0	50.0	57.9	58.1	382-517 bp	1.0	89.1
trnY-GTA-trnE-TTC_p4	GTCGAGCTGGATTTGAACCA	CTCTCTTTCAAGGAGCCGAC	50.0	55.0	58.2	58.0	388-523 bp	1.0	88.9
trnY-GTA-trnE-TTC_p5	GAAACACAACGAAGCAACCC	CTCTCTTTCAAGGAGCCGAC	50.0	55.0	58.2	58.0	733-868 bp	0.8	79.5