Hotspot

trnH-GTG-psbA

Genome · 5817-6267 · 451 bp

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Pi 0.0361

MarkerSeek score 74.40

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: Genome
length_bp: 451
variable_sites: 37
pi: 0.0361
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	TAATCCACTTGGCTACGTCC	TGAAGCTCCTTCTGTAAACGG	50.0	47.6	57.0	58.0	478-508 bp	1.0	73.1
trnH-GTG-psbA_p2	TAATCCACTTGGCTACGTCC	GAAGCTCCTTCTGTAAACGGA	50.0	47.6	57.0	57.7	477-507 bp	1.0	70.9
trnH-GTG-psbA_p3	TTAATCCACTTGGCTACGTCC	TGAAGCTCCTTCTGTAAACGG	47.6	47.6	57.7	58.0	479-509 bp	1.0	70.8
trnH-GTG-psbA_p4	CCTTAATCCACTTGGCTACGT	TGAAGCTCCTTCTGTAAACGG	47.6	47.6	57.7	58.0	481-511 bp	1.0	70.7
trnH-GTG-psbA_p5	TAATCCACTTGGCTACGTCC	AAGCTCCTTCTGTAAACGGA	50.0	45.0	57.0	56.5	476-506 bp	1.0	69.3