Hotspot

ndhI-rps15

SSC · 117487-117632 · 146 bp

Back to Trichoglottis

Pi 0.0189

MarkerSeek score 51.70

Primer available yes

Label ndhI-rps15

Feature Metadata

label_name: ndhI-rps15
feature_type: igs
region: SSC
length_bp: 146
variable_sites: 9
pi: 0.0189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-rps15_p1	AGGTAATTATGAAACTTTGTCCA	GGATCAATTAAATATTCGGGAAC	30.4	34.8	53.9	53.6	197-208 bp	1.0	47.5
ndhI-rps15_p2	AAGGTAATTATGAAACTTTGTCCA	GGATCAATTAAATATTCGGGAAC	29.2	34.8	54.7	53.6	198-209 bp	1.0	47.5
ndhI-rps15_p3	AGGTAATTATGAAACTTTGTCCAA	GGATCAATTAAATATTCGGGAAC	29.2	34.8	54.7	53.6	197-208 bp	1.0	47.5
ndhI-rps15_p4	ACAGGTAAATGATTTGTATGGA	GGATCAATTAAATATTCGGGAAC	31.8	34.8	53.1	53.6	232 bp	0.571429	22.9
ndhI-rps15_p5	ACAGGTAAATGATTTGTATGGAT	GGATCAATTAAATATTCGGGAAC	30.4	34.8	53.8	53.6	232 bp	0.571429	22.9