Hotspot

petA-psbJ

LSC · 63713-64670 · 958 bp

Back to Trichoglottis

Pi 0.00845

MarkerSeek score 55.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 24
pi: 0.00845
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCTGTTCTTCTTGGCATCGG	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.9	57.3	1074-1082 bp	1.0	75.8
petA-psbJ_p2	TCTGTTCTTCTTGGCATCGG	CTATTCCGGGTTGGGTTCAT	50.0	50.0	57.9	57.3	1075-1083 bp	1.0	75.7
petA-psbJ_p3	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.2	57.3	1087-1095 bp	1.0	75.1
petA-psbJ_p4	CTGTTCTTCTTGGCATCGGT	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.2	57.3	1073-1081 bp	1.0	75.1
petA-psbJ_p5	GTTCTTCTTGGCATCGGTGA	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.2	57.3	1071-1079 bp	1.0	75.1