Hotspot

accD-psaI

LSC · 59189-60093 · 905 bp

Back to Trichoglottis

Pi 0.0141

MarkerSeek score 53.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 34
pi: 0.0141
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AATGCCTACATTGCGTTTGC	AAAGAAGCCATTGCGATTGC	45.0	45.0	58.0	58.0	1169-1201 bp	1.0	85.4
accD-psaI_p2	AATGCCTACATTGCGTTTGC	TCAACTAGCAGCATTCCGAC	45.0	50.0	58.0	58.0	1504-1544 bp	1.0	85.3
accD-psaI_p3	CCTACAACTGGCGGAGTTAC	AAAGAAGCCATTGCGATTGC	55.0	45.0	58.0	58.0	1232-1264 bp	1.0	85.1
accD-psaI_p4	AATGCCTACATTGCGTTTGC	TTTGCTGAGACGGGAATACG	45.0	50.0	58.0	58.0	1768-1808 bp	1.0	84.7
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TTTGCTGAGACGGGAATACG	55.0	50.0	58.0	58.0	1831-1871 bp	1.0	84.5