Hotspot

atpB-rbcL

LSC · 54536-55463 · 928 bp

Back to Trichoglottis

Pi 0.0109

MarkerSeek score 58.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 28
pi: 0.0109
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGCCCTTGGTTTTGTTCTTC	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.9	58.1	1001-1066 bp	1.0	84.1
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1023-1088 bp	1.0	84.1
atpB-rbcL_p3	TACGCCCTTGGTTTTGTTCT	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.6	58.1	1003-1068 bp	1.0	82.4
atpB-rbcL_p4	CGCCCTTGGTTTTGTTCTTC	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.9	58.4	1002-1067 bp	1.0	81.7
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	1024-1089 bp	1.0	81.7