Hotspot

rps16-trnQ-UUG

LSC · 6118-6986 · 869 bp

Back to Trichoglottis

Pi 0.0107
MarkerSeek score 56.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
869
variable_sites
24
pi
0.0107
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 954-969 bp 1.0 82.1
rps16-trnQ-UUG_p2 ATCCACAGCCAATCATGTCC GACCAAAAAGAAATGGGGCG 50.0 50.0 57.9 57.9 1015-1030 bp 1.0 81.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 931-946 bp 1.0 81.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GACCAAAAAGAAATGGGGCG 45.0 50.0 58.1 57.9 992-1007 bp 1.0 81.4
rps16-trnQ-UUG_p5 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 952-967 bp 1.0 81.4