Hotspot

matK-trnK-UUU

LSC · 3256-4404 · 1149 bp

Back to Trichoglottis

Pi 0.0108

MarkerSeek score 54.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 38
pi: 0.0108
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CGGATATAGGAAGTTTTGTTGCC	TCCGGGTTGCTAACTCAATG	43.5	50.0	57.8	57.9	1249-1301 bp	1.0	57.4
matK-trnK-UUU_p2	CGGATATAGGAAGTTTTGTTGCC	CGGGTTGCTAACTCAATGGT	43.5	50.0	57.8	58.2	1247-1299 bp	1.0	56.8
matK-trnK-UUU_p3	GCGGATATAGGAAGTTTTGTTGC	TCCGGGTTGCTAACTCAATG	43.5	50.0	58.4	57.9	1250-1302 bp	1.0	55.5
matK-trnK-UUU_p4	CGGATATAGGAAGTTTTGTTGCC	GATCCGGGTTGCTAACTCAA	43.5	50.0	57.8	57.6	1251-1303 bp	1.0	55.1
matK-trnK-UUU_p5	CGGATATAGGAAGTTTTGTTGCC	TTGATCCGGGTTGCTAACTC	43.5	50.0	57.8	57.6	1253-1305 bp	1.0	55.1