Hotspot

ycf1

SSC · 127873-133467 · 5595 bp

Back to Trichocladus

Pi 0.00411

MarkerSeek score 54.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5595
variable_sites: 23
pi: 0.00411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCACTAACATTTTCAATTCCAGT	AGCCCTTTTTATCTCACAATTCA	30.4	34.8	54.5	56.4	224 bp	1.0	42.1
ycf1_p2	TCACTAACATTTTCAATTCCAGT	AGAAAAGCCCTTTTTATCTCACA	30.4	34.8	54.5	56.4	229 bp	1.0	42.1
ycf1_p3	TTCACTAACATTTTCAATTCCAGT	AGCCCTTTTTATCTCACAATTCA	29.2	34.8	55.2	56.4	225 bp	1.0	42.1
ycf1_p4	TTCACTAACATTTTCAATTCCAGT	AGAAAAGCCCTTTTTATCTCACA	29.2	34.8	55.2	56.4	230 bp	1.0	42.1
ycf1_p5	TCACTAACATTTTCAATTCCAGT	AAGCCCTTTTTATCTCACAATTCA	30.4	33.3	54.5	57.1	225 bp	1.0	42.1